Accéder au contenu
MilliporeSigma
Toutes les photos(3)

Principaux documents

Voir toute la documentation

MAK044

Sigma-Aldrich

Kit de dosage des acides gras libres

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Kit d’analyse des acides gras libres

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(3)

About This Item

Code UNSPSC :
12161503

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Application(s)

cosmetics
food and beverages

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; cancer

Température de stockage

−20°C

Description générale

Les acides gras libres, également appelés acides gras non estérifiés, circulent dans le plasma sous forme liée à l′albumine. La majorité des acides gras libres sont issus de l′alimentation ou sont mobilisés au niveau du tissu adipeux. Leur concentration plasmatique est élevée dans de nombreux troubles associés à l′obésité et ils peuvent favoriser la résistance à l′insuline dans les tissus périphériques. Des taux élevés d′acides gras libres peuvent également être associés à des pathologies telles que les sepsies et les tumeurs produisant des hormones lipoactives.

Les anomalies du métabolisme des acides gras provoquent une stéatose hépatique.[1] L′accumulation d′acides gras libres dans les myocytes génère un risque de dysfonction cardiaque.[2] Une augmentation de l′oxydation β des acides gras est impliquée dans l′ischémie cardiaque.[2]

Application

Le Kit de dosage des acides gras libres a été utilisé dans les applications suivantes :
  • Pour mesurer l′hydrolyse des lipides, en déterminant la teneur en acides gras libres dans un échantillon digéré.[3]
  • Pour mesurer les taux sériques et plasmatiques d′acides gras libres.[4][5]
  • Pour quantifier la concentration en acides gras libres de tissus hépatiques.[6]

Adéquation

Permet de détecter les acides gras libres à chaîne longue dans divers échantillons biologiques comme le sérum, le plasma et d′autres fluides corporels, les aliments, les milieux de croissance, etc.

Principe

Dans ce kit, la concentration des acides gras (en C8 et plus longs) est déterminée par un dosage enzymatique couplé, qui donne un produit mesurable par colorimétrie (570 nm) / fluorimétrie (λex = 535 nm/λém = 587 nm), dont la quantité est proportionnelle à celle des acides gras présents.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

92210

Timestrip Plus™ 0 °C

06693

Timestrip Plus™ -20 °C

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8L11K06120
8A17K06120
8C21K06120
8E18K06120
8B15K06120

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Interactions among macronutrients in wheat flour determine their enzymic susceptibility
Bhattarai R R, et al.
Food Hydrocolloids, 61, 415-425 (2016)
Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression.
A S M H Mahmood et al.
ASN neuro, 12, 1759091420910933-1759091420910933 (2020-04-03)
Changes in feed intake during isolation stress in respiration chambers may impact methane emissions assessment.
Llonch P, et al.
Animal Production Science, 58(6), 1011-1016 (2018)
Association of Temperament and acute stress responsiveness with Productivity, Feed efficiency, and Methane emissions in Beef cattle: an Observational study
Llonch P, et al.
Frontiers in veterinary science, 3, 43-43 (2016)
Sarmistha Mukherjee et al.
Hepatology (Baltimore, Md.), 65(2), 616-630 (2016-11-04)
The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of nicotinamide adenine dinucleotide (NAD) falls, at least in part due to metabolic competition for
Afficher tous les articles scientifiques apparentés

Questions

1–9 of 9 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is there a step in the process where samples can be preserved for future analysis using the kit?

    1 answer

    1. Yes, samples can be preserved for future analysis using the kit by aliquoting and freezing them if needed for multiple uses. However, it's recommended to use fresh samples whenever possible to ensure accurate results. Dried lipids can be stored in 200 µl of Fatty Acid Assay Buffer at -80°C. In case of lipid precipitation, re-dissolve the lipids by sonication. However, the supplier does not specify the maximum duration for which the samples stored at -80°C will remain stable. Storing samples at -80°C may impact their stability, potentially resulting in lower than expected readings. Therefore, it is recommended to use fresh samples for this assay whenever possible to ensure accurate results.
      If frozen samples are used, they should be snap frozen in liquid nitrogen immediately after collection. These samples cannot undergo multiple freeze-thaw cycles and must be stored for relatively short periods. It's important to note that stability studies on different sample types have not been conducted, so there is no advice available regarding the timeframe for measuring frozen samples.

      Helpful?

  4. Can MAK044 quantify only albumin-bound free fatty acids (FFA), or does it also quantify unbound FFA?

    1 answer

    1. The kit specifically detects unbound free fatty acids in the samples and does not detect any albumin-bound free fatty acids.

      Helpful?

  5. What are the recommended storage instructions for the Enhancer reagent and unused Assay Buffer in the MAK044 Free Fatty Acid Quantitation Kit?

    1 answer

    1. The enhancer can be aliquoted and stored at -20°C, while the assay buffer can also be stored in aliquots at -20°C to avoid multiple freeze-thaw cycles.

      Helpful?

  6. I would like to quantify butyric acid in bacteria culture supernatant. Does this MAK044 Free Fatty Acid Quantitation Kit suitable or is there a kit you can recommend. Thank you. 

    1 answer

    1. Butyric acid is a short-chain fatty acid. This kit is suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma, and other body fluids, food, growth media, etc. The Short Chain Fatty Acid Kit, prod number SBR0030, offers a potential analytical option. This kit is suitable for detection of several SCFAs, including Butyric, by HPLC, LC-MS, and GC-MS. See the link below to the technical bulleting should these methods be suitable:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  7. Can this kit be used to analyse NEFA in plasma using the Pentra C400 instrument?

    1 answer

    1. This kit is suitable for detecting nonesterified fatty acids in plasma (NEFA). However, suitability with Pentra C400 instrument has not been determined. This application must be determined by the end-user.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Can these kits be used with serum samples from mouse?

    1 answer

    1. Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique