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MAK022

Sigma-Aldrich

Calcium Colorimetric Assay

sufficient for 250 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 250 colorimetric tests

Application(s)

cosmetics
food and beverages

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

neurological disorders; hematological disorder

Température de stockage

2-8°C

Description générale

Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.

Application

Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Principe

In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg.

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Réf. du produit
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Tarif

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Santiago Pech-Pool et al.
International journal of molecular sciences, 21(4) (2020-02-26)
It is known that growth hormone (GH) is expressed in immune cells, where it exerts immunomodulatory effects. However, the mechanisms of expression and release of GH in the immune system remain unclear. We analyzed the effect of growth hormone-releasing hormone
Jing Che et al.
Life sciences, 182, 10-21 (2017-06-12)
The study aims to investigate the effects of Periostin gene silencing on tumor microenvironment and bone metastasis via the integrin-signaling pathway in lung cancer (LC). LC patients were divided into bone metastasis and non-bone metastasis groups; Healthy volunteers were selected
Kang Liu et al.
International journal of molecular medicine, 45(6), 1793-1802 (2020-04-03)
Calbindin‑D28K (Calb1) may protect human lens epithelial cells (HLECs) from apoptosis, which is a process resulting in individual cell death. The protective effects of Calb1 may be attributed to buffering high concentrations of Ca2+. The present study investigated the mechanisms
Calcium intake, calcium homeostasis and health.
Fan P, et al.
Food science and human wellness, 5(1), 8-16 (2016)
Creatine?facilitated protection of stress caused by disrupted circadian rhythm
George B, et al.
Biological Rhythm Research, 49(1), 61- 75 (2018)

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