M8433

Anti-phospho-c-Myc (pThr⁵⁸/pSer⁶²) antibody produced in rabbit

affinity isolated antibody, liquid

• Numéro MDL: MFCD02097158
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Poids mol.: antigen ~64-67 kDa
• Espèces réactives: rat, human, mouse
• Technique(s): immunohistochemistry: 10-20 μg/mL; immunoprecipitation (IP): 2-4 μg/mL; western blot: 0.5-4 μg/mL
• Numéro d'accès UniProt: P01106
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pThr58/pSer62)
• Informations sur le gène: human ... MYC(4609) ; mouse ... Myc(17869) ; rat ... Myc(24577)

Description

Description générale

The transcription factor c-Myc is a short-lived nuclear phosphoprotein involved in cell proliferation, differentiation, and cancer. Expression of c-Myc is strictly controlled by mitogens and is required for cell cycle entry. c-Myc binds to DNA and activates transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro at Ser⁶² by MAP Kinase, and in vivo at both Thr⁵⁸ and Ser⁶² although the pathways and kinases acting in vivo have not been identified. Mutation of Thr⁵⁸ and Ser⁶² to Ala inhibits the ability of c-Myc to stimulate transcription. c-Myc, a ~45 kDa protein that migrates with an apparent molecular weight of ~64-67kDa in reducing SDS-PAGE, appears to regulate multiple aspects of growth control; c-Myc promotes cell proliferation in the presence of Cdk inhibitors and induces apoptosis upon overexpression in serum deprived cells.

Spécificité

Does not react with non-phosphorylated c-Myc.

Immunogène

synthetic peptide corresponding to residues around phospho-Thr⁵⁸ and phospho-Ser⁶² of human c-Myc.

Forme physique

In phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Notes préparatoires

Purified by protein A and peptide affinity chromatography.

Clause de non-responsabilité

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable