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M5688

Sigma-Aldrich

Mimetic Orange 1 Ligand Affinity Adsorbent

suspension

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About This Item

Numéro MDL:
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Forme

suspension

Niveau de qualité

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Application

Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Polymerase from E. coli lysates as well as for the purification of other DNA polymerases.

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

95.0 °F - closed cup

Point d'éclair (°C)

35 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Emily R Beynon et al.
Plant physiology, 151(1), 253-261 (2009-07-17)
The explosive 2,4,6-trinitrotoluene (TNT) is a significant environmental pollutant that is both toxic and recalcitrant to degradation. Phytoremediation is being increasingly proposed as a viable alternative to conventional remediation technologies to clean up explosives-contaminated sites. Despite the potential of this
Sotirios Melissis et al.
Biotechnology journal, 2(1), 121-132 (2006-12-22)
The thermostable Thermus aquaticus DNA polymerase (Taq Pol) has been the key factor in transforming the initial PCR method into one with huge impact in molecular biology and biotechnology. Therefore, the development of effective affinity adsorbents for the purification of
Jeannie Horak et al.
Analytical and bioanalytical chemistry, 400(8), 2349-2359 (2010-12-22)
The new affinity-type Mimetic Ligand™ B14 was coupled with a 1,2-diaminoethane spacer (2LP) and a [1,2,3]-triazole spacer (TRZ) to three different support media. In addition to the agarose-based PuraBead and the polymethacrylate-type Fractogel, three new polymeric support media were introduced
S Melissis et al.
Journal of chromatography. A, 1122(1-2), 63-75 (2006-05-23)
The commercial availability of DNA polymerases has revolutionized molecular biotechnology and certain sectors of the bio-industry. Therefore, the development of affinity adsorbents for purification of DNA polymerases is of academic interest and practical importance. In the present study we describe
S J Burton
Methods in molecular biology (Clifton, N.J.), 11, 91-103 (1992-01-01)
Dye-ligand affinity chromatography is performed with an adsorbent consisting of a solid support matrix to which a dye has been cova-lently bonded. The protein mixture is passed through a packed bed of adsorbent that selectively binds proteins that are able

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