H7666

Interleukin-12 human

HumanKine^®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested

• Synonyme(s) : IL-12
• Numéro MDL: MFCD00212975
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≤2 ng/mL EC₅₀
• Poids mol.: dimer 57 kDa (glycosylated)
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Température de stockage: −20°C

Description

Application

Interleukin-12 (IL-12) human has been used to study IL-12 mediated enhancement of IL-12Rβ2 (IL-12 receptor) expression.

Actions biochimiques/physiologiques

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Forme physique

lyophilized from a 0.2 μm filtered solution of PBS

Remarque sur l'analyse

The specific activity was determined by the dose-dependent release of IFN-γ from the human NK92 cell line in presence of 20 ng/mL rIL-2.

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable