H5789

Anti-HuD antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-ELAVL4, Anti-Human Antigen D
• Numéro MDL: MFCD03791609
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 40 kDa
• Espèces réactives: human, mouse
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using heat retrieved, human intestine tissue; western blot (chemiluminescent): 5-10 μg/mL using mouse brain extract
• Numéro d'accès UniProt: P26378
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ELAVL4(1996) ; mouse ... Elavl4(15572)

Description

Description générale

Hu antigen D (HuD) protein is highly conserved and a neuron-specific RNA-binding protein. This protein belongs to the embryonic lethal, abnormal vision, Drosophila-like (ELAVL)/Hu family. The HuD gene is located on the human chromosome at 1p33-p32.3.

Spécificité

Anti-HuD specifically recognizes human and mouse HuD.

Immunogène

synthetic peptide corresponding to amino acids 240-255 of human HuD with an N-terminal added cysteine, conjugated to KLH.

Application

Anti-HuD antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.

Actions biochimiques/physiologiques

Hu antigen D (HuD) protein binds to the 3′ untranslated region (UTR) of mRNA of genes like N-myc gene and growth-associated protein (GAP43). This binding results in an increase in the half-life of these mRNA. HuD plays a vital role in several regulatory processes, such as the processing of pre-mRNA, mRNA stability and translation. This protein is also involved in neuronal functioning and development. HuD protein also plays a role in neuronal plasticity, which includes, learning and memory, recovery from axonal injury and multiple neurological diseases.

Description de la cible

HuD protein binds to the 3′ UTR of mRNA of geneslike N-myc gene and GAP43, and increases the half-life ofthese mRNA.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide and 1% inactivated bovine serum albumin.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)