GE28-9523-59

GST Spintrap^™

Cytiva 28-9523-59

• Code UNSPSC : 41106500
• Nomenclature NACRES : NA.56

Propriétés

• ligand: glutathione
• Conditionnement: pkg of 50 ea
• Fabricant/nom de marque: Cytiva 28-9523-59
• Volume du lit: 50 μL
• Matrice: 4% agarose
• Taille des particules: 45-165 μm
• Diamètre moyen: 90 μm
• cleaning: 4-13
• working range: 4-13
• Capacité: >25 mg binding capacity (horse liver GST/ml medium); ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

Description

Description générale

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

Caractéristiques et avantages

• Preserves protein antigenicity and function.
• Yields up to 500 μg of pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

SpinTrap is a trademark of Cytiva

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids