G9880

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

• Synonyme(s) : ALT1, L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
• Numéro CAS: 9000-86-6
• Numéro de classification (Commission des enzymes): 2.6.1.2 (BRENDA, IUBMB)
• Numéro Beilstein : 3562140
• Numéro CE : 232-561-4
• Numéro MDL: MFCD00131192
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Porcine heart
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥75 units/mg protein
• Poids mol.: 42 kDa
• Activité étrangère: L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%; glutamic-oxalacetic transaminase ≤0.05%
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.

Actions biochimiques/physiologiques

GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Définition de l'unité

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Forme physique

Suspension in 1.8 M (NH₄)₂SO₄ solution, pH 6.0

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)