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E0760

Sigma-Aldrich

Endothelial cell growth supplement from bovine pituitary

ECGS, suitable for cell culture, BioReagent

Synonyme(s) :

ECGS, Endothelial Mitogen

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

bovine pituitary glands

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

powder

Puissance

2-300 μg/mL ED50/EC50

Qualité

endotoxin tested

Conditionnement

pkg of 15 mg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤20 EU/vial (LAL test)

Couleur

brown to tan

Solubilité

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

Température de stockage

2-8°C

Catégories apparentées

Description générale

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:
  • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
  • as a supplement in medium 199 for endothelial cell culture
  • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Actions biochimiques/physiologiques

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H361fd

Classification des risques

Repr. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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James F Dylewski et al.
MethodsX, 7, 101048-101048 (2020-09-19)
Glomerular endothelial cells (GEnC) are a specialized microvascular subset of endothelial cells that, when injured, result in many types of diseases within the kidney. Thus, techniques to study GEnC in a cell culture system are important to investigate mechanisms of
Autoantibodies against heat shock protein 60 mediate endothelial cytotoxicity.
Schett G, et al.
The Journal of Clinical Investigation, 96(6), 2569-2577 (1995)
Pei-Yu Chou et al.
Food science & nutrition, 8(2), 1093-1103 (2020-03-10)
This study investigated the effects of methanol extract Magnolia officinalis (MEMO) on baroreceptor reflex sensitivity (BRS) in the hypercholesterolemic rabbits and the involved molecular mechanisms. Male New Zealand white rabbits were randomly divided into Control (normal diet), Cholesterol (0.5% w/w
Guihua Wu et al.
Pharmaceutical biology, 59(1), 1388-1401 (2021-10-20)
Genistein (Gen) has shown protective effects against ageing process. To explore the role of Gen on the senescence of H2O2-induced human umbilical vein endothelial cells (HUVECs) and investigate the possible mechanism. HUVECs were treated with different concentrations of H2O2 (50
TGF-beta 1 released from activated platelets can induce TNF-stimulated human brain endothelium apoptosis: a new mechanism for microvascular lesion during cerebral malaria
Wassmer SC, et al.
Journal of Immunology, 176(2), 1180-1184 (2006)
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