D5546

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• Numéro MDL: MFCD00217342
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• Nom du produit: Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Stérilité: sterile-filtered
• Forme: liquid
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Composants: NaHCO₃: yes; phenol red: yes; glucose: low; HEPES: no; sodium pyruvate: yes; L-glutamine: no
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Application

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

• human osteosarcoma cells and HeLa cells[1]
• primary human mesenchymal stromal cells (hBMSCs)[2]
• human embryonic kidney cell lines[3]

Reconstitution

Supplement with 0.584 g/L glutamine.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable