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D1821

Sigma-Aldrich

DAF-FM

≥98% (HPLC)

Synonyme(s) :

4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM

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About This Item

Formule empirique (notation de Hill):
C21H14F2N2O5
Numéro CAS:
254109-20-1
Poids moléculaire :
412.34
Code UNSPSC :
12352204
ID de substance PubChem :
329798711
Nomenclature NACRES :
NA.77

Niveau de qualité

100

Pureté

≥98% (HPLC)

Forme

powder

Conditions de stockage

desiccated

Couleur

white to brown

Solubilité

DMSO: >1 mg/mL

Conditions d'expédition

wet ice

Température de stockage

−20°C

Chaîne SMILES 

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

Clé InChI

DIJCILWNOLHJCG-UHFFFAOYSA-N

Application

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Actions biochimiques/physiologiques

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Attention

hygroscopic

Notes préparatoires

Color variation within the range from white to brown does not change performance.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings
Adavi SB, et al.
Bio-protocol, 9(20) (2019)
Phillip Roder et al.
Scientific reports, 8(1), 5892-5892 (2018-04-14)
Local manipulation of complex tissues at the single-cell level is challenging and requires excellent sealing between the specimen and the micromanipulation device. Here, biological applications for a recently developed loading technique for a force- and pressure-controlled fluidic force microscope micropipette
Judy Choi et al.
Environmental health perspectives, 118(5), 589-595 (2010-05-05)
Silica nanoparticles (SiNPs) are being formulated for cellular imaging and for nonviral gene delivery in the central nervous system (CNS), but it is unclear what potential effects SiNPs can elicit once they enter the CNS. As the resident macrophages of
Nobuo Ueda et al.
Scientific reports, 6, 37546-37546 (2016-11-23)
In many marine invertebrates, larval metamorphosis is induced by environmental cues that activate sensory receptors and signalling pathways. Nitric oxide (NO) is a gaseous signalling molecule that regulates metamorphosis in diverse bilaterians. In most cases NO inhibits or represses this
Hongwei Gao et al.
Cell death & disease, 8(8), e3004-e3004 (2017-08-18)
Tanshinones belong to a group of lipophilic constituents of Salvia miltiorrhiza Bunge (Danshen), which is widely used in traditional Chinese medicine. A deluge of studies demonstrated that tanshinones exert anti-inflammatory effects, but the underlying mechanisms remain unclear to date. This
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