The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.
CS0010
β-Secretase (BACE1) Activity Detection Kit (Fluorescent)
1 kit sufficient for 250 reactions
Synonyme(s) :
BACE1 Activity Detection Kit (Fluorescent)
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CS0010-1KT
1 840,00 $
1 840,00 $
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A propos de cet article
NACRES:
NA.84
UNSPSC Code:
12161503
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kit sufficient for 250 reactions
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... BACE1(23621)
Catégories apparentées
1 of 4
Cet article | |||
|---|---|---|---|
| Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) |
| usage kit sufficient for 250 reactions | usage - | usage - | usage - |
| Quality Level 300 | Quality Level 200 | Quality Level - | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Application
The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.
Biochem/physiol Actions
BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.
Composants de kit seuls
Réf. du produit
Description
- Fluorescent Assay Buffer 50 mL
- Stop Solution 15 mL
- Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
- Assay Standard 140 μL
- BACE1 (β−Secretase) 300 units 100 μL
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
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what is the concentration of BACE1 enzyme in micromolar?
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