CG200
cGMP Enzyme Immunoassay Kit, Direct
sufficient for 96 assays
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 96 assays
Niveau de qualité
Technique(s)
ELISA: suitable
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... PRKG1(5592) , PRKG2(5593)
Application
Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.
Composants de kit seuls
Réf. du produit
Description
- 0.1 M Hydrochloride Acid 1 x 30
- Acetic Anhydride 1 x 1
- cGMP-Alkaline Phosphatase Conjugate 1 x 5
- cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
- Cyclic GMP Standard 1 x 0.5
- Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
- Neutralizing Reagent 1 x 6
- p-Nitrophenyl Phosphate Substrate Solution 1 x 20
- Plate Sealer 1 ea
- Stop Solution 1 x 5
- Triethylamine 1 x 2
- Wash Buffer Concentrate 1 x 30
Afficher tout (12)
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
Organes cibles
Respiratory system
Risques supp
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
120.2 °F
Point d'éclair (°C)
49 °C
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Madiha A H Hassan et al.
BMC pharmacology, 5, 10-10 (2005-04-09)
Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
Nadežda Lukáčová et al.
Frontiers in physiology, 11, 700-700 (2020-07-14)
We previously reported NO/sGC signaling in the upper respiratory pathway, receiving input from the respiratory neurons of the brainstem to phrenic motoneurons in the C3-C6 spinal cord. In order to assess whether innervation of the neuromuscular junction (NMJ) at the
Lance M Wheeler et al.
Nature communications, 8(1), 1722-1722 (2017-11-25)
Materials with switchable absorption properties have been widely used for smart window applications to reduce energy consumption and enhance occupant comfort in buildings. In this work, we combine the benefits of smart windows with energy conversion by producing a photovoltaic
Yuichiro Kunai et al.
Journal of the American Chemical Society, 139(43), 15328-15336 (2017-10-08)
The concept of electrical energy generation based on asymmetric chemical doping of single-walled carbon nanotube (SWNT) papers is presented. We explore 27 small, organic, electron-acceptor molecules that are shown to tune the output open-circuit voltage (V
Frank Gaupels et al.
Frontiers in plant science, 7, 154-154 (2016-02-24)
Cucurbits developed the unique extrafascicular phloem (EFP) as a defensive structure against herbivorous animals. Mechanical leaf injury was previously shown to induce a systemic wound response in the EFP of pumpkin (Cucurbita maxima). Here, we demonstrate that the phloem antioxidant
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