LB253

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human blood
• Conditionnement: tube of 5 μg 98062330-DNA-5UG; pkg of vial of cells 98062330-1VL
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblastoid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lymphocytes, Xeroderma pigmentosum group C, EBV-transformed

Description de la lignée cellulaire

Derived from mononuclear cells from the peripheral blood of a 67 year old male. The patient was diagnosed with Xeroderma pigmentosum group C and showed spontaneous regression of melanomas. A normal level of natural killer (NK) cell activity and number, despite marked lymphopenia, was reported. The original sample was used in UV irradiation studies where UV-sensitivity of lymphocytes and fibroblasts was detected. In addition, a defect in excision repair was reported.

Application

DNA repair deficiency studies, hprt mutant analysis

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 17,19

Milieu de culture

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Procédure de repiquage

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Autres remarques

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