KYSE-30

94072011, human esophagus, Polygonal

• Synonyme(s) : KYSE30 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: KYSE-30, 94072011
• Source biologique: human esophagus
• Mode de croissance: Adherent
• Caryotype: Aneuploid
• Morphologie: Polygonal
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Asian squamous cell carcinoma

Description de la lignée cellulaire

KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.

Application

Study of human oesophageal cancer.

Milieu de culture

RPMI 1640 + Ham′s F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.