ATHOS

93122325, Porcine thyroid, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: ATHOS, 93122325
• Source biologique: Porcine thyroid
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Thyroglobulin, plasminogen activator (u-PA)
• Récepteurs: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Porcine thyroid epithelial

Description de la lignée cellulaire

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

Application

Mechanism of thyroid follicle reconstruction

Milieu de culture

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Autres remarques

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