SW 1417

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102543, human colon, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human colon
• Conditionnement: tube of 5 μg 90102543-DNA-5UG; pkg of vial of cells 90102543-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 46
• Morphologie: Epithelial
• Produits: Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human colon adenocarcinoma

Description de la lignée cellulaire

Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.

Application

Tumourigenicity studies

Milieu de culture

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Centrifugation and resuspension are recommended to disperse clumps.

Autres remarques

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