DHD/K12/TRb

90062901, rat colon, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: DHD/K12/TRb, 90062901
• Source biologique: rat colon
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat colonic carcinoma

Description de la lignée cellulaire

This cell line has properties essential for study of metastasis of colon carcinomas; a reproducible pattern of metastatic behaviour and ability to be propagated both in vitro and in vivo to facilitate identification of biochemical correlates of metastasis.

Application

Metastasis studies

Milieu de culture

DMEM + F10 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or 2% Rat Serum.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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