MiCL1 (S+L-)

86020603, mink lung, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• product name: MiCL1 (S+L-), 86020603
• Source biologique: mink lung
• Mode de croissance: Adherent
• Caryotype: 2n = 30
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mink lung, Moloney murine sarcoma virus infected

Description de la lignée cellulaire

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.

Application

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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