CHO Cell Line from Chinese hamster ovary

85050302, hamster ovary, Epithelial

• Synonyme(s) : CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• Code UNSPSC : 41106514

Propriétés

• product name: CHO Cell Line from Chinese hamster ovary, 85050302, from hamster ovary
• Source biologique: hamster ovary
• Disponibilité: not available in Japan
• Mode de croissance: Adherent
• Caryotype: Hypodiploid, modal no. 20
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Hamster Chinese ovary

Description de la lignée cellulaire

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

Application

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

Milieu de culture

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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