Primary mouse embryonic fibroblasts (MEFs) were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13.5 post coitus. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by a standard serial passaging protocol.
Milieu de culture
DMEM + 10% FCS + 2mM Glutamine
Procédure de repiquage
Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10,000 cells per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37oC
Autres remarques
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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Clause de non-responsabilité
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Lot/Batch Number
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