C9205

Anticorps monoclonal anti-protéine acide fibrillaire gliale (GFAP) antibody produced in mouse

clone G-A-5, purified from hybridoma cell culture

• Synonyme(s) : Anticorps anti-GFAP
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: CY3 conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: G-A-5, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human, pig, rat
• Technique(s): direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections
• Isotype: IgG1
• Numéro d'accès UniProt: P14136
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GFAP(2670) ; rat ... Gfap(24387)

Description

Description générale

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Spécificité

L′anticorps réagit spécifiquement avec GFAP dans les essais d′immunoblotting et permet de marquer les astrocytes, les cellules gliales de Bergmann et les chondrocytes de cartilage élastique en coloration immunohistochimique. L′anticorps réagit avec un antigène spécifique des cellules gliales dans les sections de tissus congelés ou fixés à l′alcool.

The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Immunogène

GFAP de moelle épinière de porc

GFAP from pig spinal cord

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in

• immunohistochemistry
• immunoblotting
• immunolabelling,
• immunocytochemistry
• immunofluorescence

Actions biochimiques/physiologiques

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Informations légales

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)