The media provided by Cell Applications contains a proprietary complete formulation. For the Complete Media, the final supplement concentrations (after addition to the medium) are as follows:
- Fetal Calf Serum: 0.04 ml/ml
- Bovine Pituitary Extract: 0.004 ml/ml
- Basic Fibroblast Growth Factor (recombinant human): 1 ng/ml
- Insulin (recombinant human): 5 μg/ml
For further details, please refer to the Datasheet [here](https://promocell.com/wp-content/uploads/product-information/manual/C-26500.pdf). If you need to determine the presence or absence of a particular component or respective concentration not mentioned above, please contact Technical Service with justification, and a request for the relevant information can be made. However, it's important to note that there is no guarantee that this information will be provided.