PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
C-12980
Human Pericytes from Placenta (hPC-PL)
500,000 cryopreserved cells
Synonyme(s) :
Pericyte Culture
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About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81
2 400,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Source biologique
human placenta
Conditionnement
pkg of 500,000 cells
Morphologie
( pericytes)
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
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Origine de la lignée cellulaire
Placenta
Application
Human Pericytes are isolated from human placenta tissue and provided in a cryopreserved format. Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas. To date, pericytes have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
Qualité
Rigid quality control tests are performed for each lot of Pericytes. The cells are tested for morphology, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis for a comprehensive panel ofmarkers, e.g. CD31, CD34, CD105, and CD146. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Avertissement
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Procédure de repiquage
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Autres remarques
Recommended Plating Density: 4000 cells per cm2Tested markers: CD146+, CD105+, CD31-, CD34-
Produits recommandés
Recommended Primary Cell Culture Media:Link
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Articles
What are pericytes? What are common pericyte markers? Are pericytes mesenchymal stem cells? Learn more about pericytes in our FAQ page. Explore over 350 PromoCell products.
Primary human pericytes and cell culture media optimized for the in vitro cultivation of human pericytes from all tissues. Protocols for handling, cell passaging, media, and product use.
Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.
Protocoles
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
Contenu apparenté
Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.
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How long do pericytes grow in culture?
1 answer-
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What markers are used to characterize pericytes?
1 answer-
Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
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How are pericytes isolated?
1 answer-
Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
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