A5268

Alcian Blue 8GX

powder

• Synonyme(s) : Alcian Blue, Ingrain Blue 1
• Formule empirique (notation de Hill): C₅₆H₆₈Cl₄CuN₁₆S₄
• Numéro CAS: 33864-99-2
• Poids moléculaire : 1298.86
• Numéro C.I. (Colour Index): 74240
• Numéro CE : 251-705-7
• Numéro MDL: MFCD00010720
• Code UNSPSC : 12171500
• ID de substance PubChem : 24890962
• Nomenclature NACRES : NA.47

Propriétés

• Forme: powder
• Niveau de qualité: 100
• Composition: Dye content, 45-65%
• Technique(s): microbe id | staining: suitable
• Couleur: blue
• Solubilité: H₂O: 1 mg/mL
• εmax: 425 at 615-630 nm in water
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
• InChI: 1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
• Clé InChI: CKLBXIYTBHXJEH-UHFFFAOYSA-J

Description

Description générale

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.[1]

Application

• Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.[2][3]
• It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.[4]
• It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.[5]

Caractéristiques et avantages

Desired staining patterns can be obtained by manipulating pH and salt concentrations.[1]

Principe

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)