After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.
A25120
DEAE Sephadex® A-25 chloride form
Synonyme(s) :
DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®
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10 G
198,00 $
50 G
705,00 $
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10 G
198,00 $
50 G
705,00 $
About This Item
198,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Forme
powder
Niveau de qualité
Technique(s)
activity assay: suitable
Matrice
cross-linked dextran
Groupe de la matrice active
functional group
Taille des billes
40-125 μm (dry)
Dimension de pores
~30,000 Da exclusion limit
pH de fonctionnement
2-9
Capacité
3-4 meq/g ion exchange capacity
Compatibilité
mode of use mixed weak and strong anion exchange chromatography
Description générale
The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
Application
DEAE-Sephadex® A-25 has been used:
- to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
- in the estimation of GLS content in Chinese kale sprouts
- production and purification of endozepine from astrocytes
- to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients
Caractéristiques et avantages
- Weak anion exchanger
- Suitable for batch techniques
- High binding capacities
- Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride
Informations légales
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
Remplacé(e)(s) par
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
T M Parkinson
Journal of lipid research, 8(1), 24-29 (1967-01-01)
Various cellulose and dextran anion exchangers bind bile salts in vitro under conditions of pH and ionic strength resembling those in the lumen of the small intestine. Of these substances, diethylaminoethyl (DEAE) cellulose, guanidoethyl cellulose, and DEAE Sephadex reduced hypercholesterolemia
Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Hohn, D., et al.
Immunological Investigations, 12(5), 481-490 (1983)
A C Newton et al.
Analytical biochemistry, 156(1), 56-60 (1986-07-01)
The greatly enhanced fluorescence of Tb3+ when complexed with dipicolinic acid affords a simple and highly sensitive method for monitoring continuous anion flux through the erythrocyte anion transporter, band 3. Dipicolinic acid (pyridine-2,6-dicarboxylic acid) is entrapped in human erythrocytes and
In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
P H Jones
Experimental cell research, 139(2), 401-404 (1982-06-01)
Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
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How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should I store Product A25120, DEAE-Sephadex® A-25 chloride?
1 answer-
Unused media can be stored in the container at +4 to +30 °C. Ensure that the screw-top is fully tightened. Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.
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How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.
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What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.
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