The typical final working concentrations for cell loading is in the range of 1 to 10 micromolar. It is best to find literature references dealing with your application for more information on the amounts to use.
A1076
BAPTA-AM
≥95% (HPLC), powder, intracellular Ca²⁺ store chelator
Synonyme(s) :
1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
25 MG
367,00 $
Sélectionner une taille de conditionnement
Changer de vue
25 MG
367,00 $
About This Item
Formule empirique (notation de Hill) :
C34H40N2O18
Numéro CAS:
Poids moléculaire :
764.68
Beilstein:
7458534
Numéro MDL:
Code UNSPSC :
12352200
ID de substance PubChem :
Nomenclature NACRES :
NA.77
Produits recommandés
Nom du produit
BAPTA-AM, ≥95% (HPLC)
Niveau de qualité
Essai
≥95% (HPLC)
Forme
powder
Température de stockage
−20°C
Chaîne SMILES
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccccc1OCCOc2ccccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O
InChI
1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3
Clé InChI
YJIYWYAMZFVECX-UHFFFAOYSA-N
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Application
BAPTA-AM has been used :
- to reduce the elevation of cleaved caspase-3[3]
- to block cell death, inhibit ROS (reactive oxygen species) production and inhibit caspase-8 activation[4]
- to exclude the effect of changes in intracellular Ca2+ ([Ca2+]i) concentration during seizure induction[5]
- to eliminate the increase of [Ca2+]i induced by 6-hydroxydopamine (6-OHDA)[6]
Actions biochimiques/physiologiques
Selective chelator of intracellular Ca2+ stores.
Liaison
Membrane-permeable form of BAPTA.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Gloves
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures
Wie MB, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 25(8), 1641-1659 (2001)
Glycolysis is essential for chemoresistance induced by transient receptor potential channel C5 in colorectal cancer
Wang T, et al.
BMC Cancer, 18(1), 207-207 (2018)
Blockade of RyRs in the ER Attenuates 6-OHDA-induced calcium overload, cellular hypo-excitability and apoptosis in dopaminergic neurons
Huang L, et al.
Frontiers in Cellular Neuroscience, 11, 52-52 (2017)
The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells
Tang Q, et al.
Biochemical Pharmacology, 74(11), 1596-1607 (2007)
Hyperactivation of the mammalian degenerin MDEG promotes caspase-8 activation and apoptosis
Pan JA, et al.
The Journal of Biological Chemistry, 288(5), 2952-2963 (2013)
Articles
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
-
What is the typical working concentration for BAPTA-AM, Product A1076?
1 answer-
Helpful?
-
-
How would you recommend that I store my BAPTA-AM, Product A1076, solution?
1 answer-
Stock solutions of 1-10 mM can be prepared in anhydrous DMSO, divided into single-use aliquots, and stored desiccated and frozen at -20°C.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What are the shipping and storage temperatures for BAPTA-AM, Product A1076?
1 answer-
BAPTA-AM is shipped at ambient temperature. The recommended storage temperature, once received, is -20 °C.
Helpful?
-
-
What is BAPTA-AM, Product A1076, and how is it used?
1 answer-
BAPTA-AM is a cell-permeant acetoxymethyl ester derivative of BAPTA (Product No. A4926), which is used as a chelator of intracellular Ca2+ stores. The acetoxymethyl ester derivative, which is an ester of the carboxylic acid groups in BAPTA, results in an uncharged molecule which allows it to penetrate cell membranes. Inside the cell, these groups are cleaved by nonspecific esterase activity resulting in the free acid form.
Helpful?
-
-
In what solvents is BAPTA-AM, Product A1076, soluble?
1 answer-
BAPTA-AM is soluble in dimethyl sulfoxide (DMSO) at 25 mg/mL. It is also soluble in acetonitrile at 0.5 mg/mL. It is best to use anhydrous DMSO, since it is known that moisture uptake by the DMSO can adversely affect the quality of the BAPTA-AM.
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
