94072246
RPMI 7932 cell line
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1 VIAL
1 350,00 $
1 350,00 $
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1 VIAL
1 350,00 $
About This Item
Code UNSPSC :
41106514
1 350,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Source biologique
human lung (effusion)
Forme
liquid
Conditionnement
tube of 5 μg 94072246-DNA-5UG
pkg of vial of cells 94072246-1VL
Mode de croissance
Adherent
Caryotype
Not specified
Morphologie
Epithelial-like
Produits
Plasminogen activator
Récepteurs
Not specified
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
cancer
Origine de la lignée cellulaire
Human pleural effusion from a patient with malignant melanoma
Description de la lignée cellulaire
Derived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.
Application
Tumourigenicity studies
Conditionnement
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Milieu de culture
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Procédure de repiquage
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Autres remarques
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Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
J A Eisman et al.
Cancer research, 47(1), 21-25 (1987-01-01)
It has been demonstrated previously that several human cancer cell lines possess specific, high affinity receptors for 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3, calcitriol] and that 1,25-(OH)2D3 and certain of its metabolites inhibit the growth in vitro of several human breast cancer and
C W Porter et al.
Cancer research, 54(22), 5917-5924 (1994-11-15)
Certain N-alkylated analogues of the natural polyamine spermine, such as N1,N11-diethylnorspermine (DENSPM), rapidly deplete intracellular polyamine pools by down-regulating the biosynthetic enzymes, ornithine decarboxylase and S-adenosylmethionine decarboxylase, and by potently up-regulating the polyamine catabolizing enzyme, spermidine/spermine N1-acetyltransferase. On the basis
B Sharma
Cancer research, 37(12), 4660-4668 (1977-12-01)
Tumor cell fractions isolated from tumor lines SH-3 (breast carcinoma) and RPMI-7932 (malignant melanoma) by differential centrifugations were capable of transforming lymphocytes into cytotoxic effector cells. Lymphocytes cultured alone in human AB plasma did not become cytotoxic to tumor cells.
Generation of cytotoxic lymphocytes against human tumor cells in vitro by various soluble microbial extracts.
B Sharma et al.
Journal of the National Cancer Institute, 63(2), 341-346 (1979-08-01)
B K Bhuyan et al.
Cancer research, 46(4 Pt 1), 1688-1693 (1986-04-01)
Our interest in prostaglandins (PGs) as antitumor agents stemmed from the report of Bregman and Meyskens (Cancer Res., 43: 1642-1645, 1983) that PGA1, PGA2, and PGD2 inhibited colony formation by human melanoma cells obtained from fresh biopsies of melanoma patients.
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