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Sigma-Aldrich

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt

≥98.0% (TLC)

Synonyme(s) :

Biotin-X-NTA

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About This Item

Formule empirique (notation de Hill):
C26H40K3N5O9S
Numéro CAS:
856661-92-2
Poids moléculaire :
715.98
Code UNSPSC :
12352209
Nomenclature NACRES :
NA.32

Pureté

≥98.0% (TLC)

Forme

solid

Chaîne SMILES 

[K+].[K+].[K+].[O-]C(=O)CN(CC([O-])=O)[C@H](CCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C([O-])=O

Application

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine (Biotin-X-NTA) is used as a hetero-bifunctional linker between histone(His)-tagged proteins and (strept)avidin conjugates.
Hetero-bifunctional linker for His-tagged proteins on one side and (strept)avidin conjugates

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Eunah Kang et al.
Langmuir : the ACS journal of surfaces and colloids, 23(11), 6281-6288 (2007-04-21)
Silica surfaces modified with nitrilotriacetic acid (NTA)-polyethylene glycol (PEG) derivatives were used to immobilize hexahistidine-tagged green fluorescent protein (His6-GFP), biotin/streptavidin-AlexaFluor555 (His6-biotin/SA-AF), and gramicidin A-containing vesicles (His6-gA). Three types of surface-reactive PEG derivatives-NTA-PEG3400-Si(OMe)3, NTA-PEG3400-vinylsulfone, and mPEG5000-Si(OMe)3 (control)-were grafted onto silica and
Xiue Jiang et al.
Physical chemistry chemical physics : PCCP, 10(42), 6381-6387 (2008-10-31)
Surface enhanced infrared absorption spectroscopy (SEIRAS) has been employed to monitor the orientated assembly of a strep-tagged membrane protein on the gold surface via a streptavidin/biotin interlayer. The high surface sensitivity of SEIRAS allows for tracking the individual assembling steps
Igor L Medintz et al.
Langmuir : the ACS journal of surfaces and colloids, 21(12), 5501-5510 (2005-06-01)
This report describes two related methods for decorating cowpea mosaic virus (CPMV) with luminescent semiconductor nanocrystals (quantum dots, QDs). Variants of CPMV are immobilized on a substrate functionalized with NeutrAvidin using modifications of biotin-avidin binding chemistry in combination with metal
S A McMahan et al.
Analytical biochemistry, 236(1), 101-106 (1996-04-05)
Using a one-step reaction, a bifunctional compound was synthesized for detecting histidine-tagged proteins immobilized on nitrocellulose. This compound has a biotin as one functional group and a nitrilotriacetic acid as the other. The nitrilotriacetic acid is used to chelate a
D J O'Shannessy et al.
Analytical biochemistry, 229(1), 119-124 (1995-07-20)
The use of short peptide affinity tag sequences has become commonplace for the expression and purification of recombinant proteins. Many of these tags are antibody epitopes and detection of tagged proteins via Western blots is straightforward. However, the most common
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