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41155

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® CAGE 500 antibody produced in goat

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

Synonyme(s) :

Abberior® CAGE 500P-Anti-Rabbit IgG antibody produced in goat

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.45

Source biologique

goat

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

rabbit

Disponibilité

available only in USA and Canada

Concentration

~1 mg/mL

Fluorescence

λex 511 nm; λem 525 nm in PBS, pH 7.4 (photoactivated)

Adéquation

corresponds for SDS-PAGE

Température de stockage

−20°C

Description générale

Abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which fluoresces at 511 nm.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)

Adéquation

Designed and tested for fluorescent super-resolution microscopy

Remarque sur l'analyse

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Informations légales

abberior is a registered trademark of Abberior GmbH

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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