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Millipore

Bolton Broth, Base

suitable for microbiology, NutriSelect® Plus

Synonyme(s) :

Bolton Selective Enrichement Broth, Base

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About This Item

Code UNSPSC :
41171607
Nomenclature NACRES :
NA.85

Agence

according to ISO 10272-1:2017

Niveau de qualité

Stérilité

non-sterile

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Composition

enzymatic digest of animal tissues, 10 g/L
α-ketoglutaric acid, 1.0 g/L
lactalbumin hydrolysates, 5.0 g/L
sodium carbonate, 0.6 g/L
sodium chloride, 5.0 g/L
sodium metabisulfite, 0.5 g/L
sodium pyruvate, 0.5 g/L
yeast extract, 5.0 g/L

Fabricant/nom de marque

NutriSelect® Plus

Technique(s)

microbiological culture: suitable

pH final

7.4±0.2 (25 °C)

Application(s)

clinical testing
food and beverages
pathogen testing
sample preparation

microbiology

Adéquation

selective for Campylobacter spp.

Description générale

Bolton broth base is used for the selective enrichment of Campylobacter species from foods as recommended by ISO Committee under specifications ISO 10272-1:2006 (E).

Application

for the selective enrichment of Campylobacter species from foods

Notes préparatoires

Suspend 13.8 grams in 472.5 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1vial of Bolton Selective Supplement (Cat. No. 40568) and 25 ml of sterile lysed defribinated horse blood. Mix well before dispensing into sterile tubes.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

CorrosionExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Dam. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves


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Certificats d'analyse (COA)

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Articles

Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.

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