ST1711

Anti-DFHR Mouse mAb (2B10)

liquid, clone 2B10, Calbiochem^®

• Synonyme(s) : Anti-Dihydrofolate Reductase
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2B10, monoclonal
• Forme: liquid
• Ne contient pas: preservative
• Espèces réactives: rat, human
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Isotype: IgG2a
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DHFR(1719)

Description

Description générale

Purified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein.

Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells.

This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR.

Immunogène

A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

Avertissement

Toxicity: Regulatory Review (Z)

Forme physique

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Negative Control
293T cells

Positive Control
HeLa cells, PC-12 Cells

Autres remarques

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Vodenicharov, M.D., et al. 2010. The EMBO Journal29, 3007.
Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol.295 H2178.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable