MABT495

Anti-MYL3, clone 7C1 Antibody

ascites fluid, clone 7C1, from mouse

• Synonyme(s) : Myosin light chain 3, Cardiac myosin light chain 1, CMLC1, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MLC1SB, Ventricular/slow twitch myosin alkali light chain
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 7C1, monoclonal
• Espèces réactives: rat
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: P08590
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: rat ... Myl3(24585)

Description

Description générale

Myosin light chain 3 (MYL3), also known as Cardiac myosin light chain 1 (CMLC1) or Myosin light chain 1 slow-twitch muscle B/ventricular isoform (MLC1SB) or Ventricular/slow twitch myosin alkali light chain (VLC1) functions as a regulatory light chain of myosin. Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.

Immunogène

Purified recombinant fragment of human MYL3 expressed in E. Coli

Application

Detect MYL3 using this Anti-MYL3, clone 7C1 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MYL3 in human skeletal muscle and cardiac muscle tissues.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualité

Evaluated by Western Blotting in rat fetal heart tissue lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected MYL3 in rat fetal heart tissue lysate.

Description de la cible

~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1