MABT163

Anti-γ-Tubulin Antibody

mouse monoclonal, 6H3.1

• Synonyme(s) : Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Nom du produit: Anti-Gamma-1-Tubulin, clone 6H3.1 Antibody, clone 6H3.1, from mouse
• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6H3.1, monoclonal
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001061
• Numéro d'accès UniProt: P23258
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TUBG1(7283)

Description

Description générale

Gamma-1-Tubulin, also known as Gamma-tubulin complex component 1, GCP-1 or Tubulin gamma-1 chain, and encoded by the gene TUBG1/TUBG, is a form of tubulin protein and a component of microtubules. Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome and plays a major role in the organization of the microtubule cytoskeleton. Gamma-1-Tubulin is part of the pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, and it is also involved in centrosome duplication and spindle formation. Interestingly, gamma tubulin is often up regulated but improperly compartmentalized in many cancer cells including non-small cell lung cancer. Mutations in Gamma-1-Tubulin gene are associated with developmental cortical defects including microcephaly and malformations of cortical development (MCD). Gamma-1-Tubulin is localized to the cytoplasm and cytoskeleton components and expressed in a variety of cell types and tissues.

Immunogène

GST-tagged recombinant protein corresponding to human Gamma-1-Tubulin.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Gamma-1-Tubulin in NIH/3T3 cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

This Anti-Gamma-1-Tubulin antibody, clone 6H3.1 is validated for use in western blotting & ICC for the detection of Gamma-1-Tubulin.

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Gamma-1-Tubulin in 10 µg of HeLa cell lysate.

Description de la cible

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable