MABT1549
Anti-MT5-MMP/MMP24 Antibody
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Conjugué:
unconjugated
application:
WB
Clone:
3D2 also termed M24N 3D2, monoclonal
Espèces réactives:
mouse, human
citations:
Technique(s):
western blot: suitable
Source biologique
rat
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
3D2 also termed M24N 3D2, monoclonal
Poids mol.
calculated mol wt 70.46 kDa
Espèces réactives
mouse, human
Conditionnement
antibody small pack of 100 μg
Technique(s)
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Mmp24(17391)
Description générale
Matrix metalloproteinase-24 (UniProt: Q9R0S2; also known as MMP-24, Matrix metalloproteinase-21, MMP-21, Membrane-type matrix metalloproteinase 5, MT-MMP 5, MTMMP5, Membrane-type-5 matrix metalloproteinase, MT5-MMP, MT5MMP) is encoded by the Mmp24 (also known as Mmp21, Mt5mmp) gene (Gene ID: 17391) in murine species. MMP-24 is a single-pass type I membrane protein that is mainly expressed in neuronal cells of both central and peripheral nervous systems. It mediates the cleavage of N-cadherin and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. It is also involved in cell-cell interactions between nociceptive neurites and mast cells. MMP-24 is synthesized with a signal peptide (aa 1-41) and a propeptide (aa 42-128) that are subsequently cleaved off to generate functional MMP-24 (aa 129-618) or processed MMP-24 (aa 129-554). MMP-24 contains a cysteine switch motif (aa 110-117) that binds the catalytic zinc ion and inhibits its enzyme activity. The dissociation of the cysteine from the zinc ion upon the activation-peptide release results in the activation of enzyme.
Spécificité
Clone 3D2 is a rat monoclonal antibody that detects MT5-MMP/MMP-24. It targets an epitope within 16 amino acids from the extracellular domain.
Immunogène
Ovalbumin-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human MT5-MMP.
Application
Quality Control Testing
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Tested Applications
Western Blotting Analysis: A representative lot detected MT5-MMP/MMP24 in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).
Western Blotting Analysis: A 1:200 dilution from a representative lot detected MT5-MMP/MMP24 in lysate from HEK293 cells over expressing MT5-MMP, but not in HEK293 cells over expressing MT1-MMP and in mock control HEK293 cell lysates (Negative). (Courtesy of Michael Willem, Biomedical Center, Ludwig Maximilian University of Munich, Germany).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Tested Applications
Western Blotting Analysis: A representative lot detected MT5-MMP/MMP24 in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).
Western Blotting Analysis: A 1:200 dilution from a representative lot detected MT5-MMP/MMP24 in lysate from HEK293 cells over expressing MT5-MMP, but not in HEK293 cells over expressing MT1-MMP and in mock control HEK293 cell lysates (Negative). (Courtesy of Michael Willem, Biomedical Center, Ludwig Maximilian University of Munich, Germany).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MT5-MMP/MMP24, clone 3D2, Cat. No. MABT1549, is a rat monoclonal antibody that detects MT5-MMP and is tested for use in Western Blotting.
Forme physique
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at +2°C to +8°C from date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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