MABS1720

Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2

clone 6C1.2, from mouse

• Synonyme(s) : Acetyl-coenzyme A synthetase, cytoplasmic, Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6C1.2, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_061147
• Numéro d'accès UniProt: Q9NR19
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ACSS2(55902)

Description

Description générale

Acetyl-coenzyme A synthetase, cytoplasmic (EC 6.2.1.1; UniProt Q9NR19; also known as Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2) is encoded by the ACSS2 (also known as ACAS2) gene (Gene ID 55902) in human. ACS plays a vital role in prokaryotic and eukaryotic cells by activating acetate to form acetyl CoA that serves as a source of two carbon metabolite for lipid synthesis and energy producing reactions. It is also reported to play a role in gene expression. In yeast cells it delivers acetyl CoA for histone acetylation. ACS contributes to cancer cell growth under low-oxygen and lipid-depleted conditions and its expression is upregulated under metabolically stress. Silencing ACSS2 gene is shown to reduce the growth of tumor xenografts. Ref.: Starai, VJ., and Escalante-Semerena, JC. (2004). Cell. Mol. Life Sci. 61, 2020 2030. Schug, ZT et al. (2015). Cancer Cell. 27:57-71.

Spécificité

Immunogen sequence is present in both spliced isoforms of human acetyl-CoA synthetase reported by UniProt (Q9NR19).

Immunogène

GST-tagged recombinant human acetyl-CoA synthetase N-terminal fragment.

Application

Detect Acetyl-CoA synthetase using this mouse monoclonal Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2, Cat. No. MABS1720, validated for use in Western Blotting.

Qualité

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl-CoA synthetase in 10 µg of HepG2 cell lysate.

Description de la cible

~80 kDa observed. 78.58/80.09 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable