MABN841

Anti-Semaphorin 4A Antibody, clone 8B5.1

clone 8B5.1, from mouse

• Synonyme(s) : Semaphorin 4A, Semaphorin-B, Sema B, Sema4A
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 8B5.1, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgMκ
• Numéro d'accès NCBI: NP_001180229
• Numéro d'accès UniProt: Q9H3S1
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SEMA4A(64218)

Description

Description générale

Semaphorin-4A (UniProt Q9H3S1; also known as Sema B, Semaphorin-B) is encoded by the SEMA4A (also known as CORD10, RP35, SEMAB, SEMB) gene (ORP UNQ783/PRO1317; Gene ID 64218) in human. Semaphorin-4A is a membrane-type class IV semaphorin originally characterized for its role in axon guidance during neural development, Semaphorin-4A also plays a role in Th cells activation and the differentiation of Th1 and Th17 cells. In addition Semaphorin-4A is involved in angiogenesis and endothelial cells migration by modulating VEGF signaling. Consistent with its important role in Th cells, Semaphorin-4A level is reported to be elevated in the serum samples from patients with multiple sclerosis (MS) respond poorly to interferon-beta (IFN-β) therapy. Semaphorin-4A neutralizing antibodies are reported to inhibit antigen-specific T cell generation and ameliorate experimental autoimmune encephalomyelitis (EAE), an animal model of MS.

Spécificité

Expected to react with both spliced isoforms.

Immunogène

Epitope: Sema domain

GST-tagged recombinant protein corresponding to the Sema domain of human Semaphorin 4A.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Semaphorin 4A Antibody, clone 8B5.1 is validated for use in Western Blotting for the detection of Semaphorin 4A.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Semaphorin 4A in 10 µg of human retinal pigment epithelia (RPE) ARPE-19.

Qualité

Evaluated by Western Blotting in human monocytic leukemia THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Semaphorin 4A in 10 µg of human monocytic leukemia THP-1 cell lysate.

Description de la cible

~85 kDa observed. 83.6 kDa (isoform 1) and 69.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Forme physique

Format: Purified

Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable