MABE866
Anti-Mad2 Antibody, clone AS55-A12
clone AS55-A12, from mouse
Synonyme(s) :
Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
AS55-A12, monoclonal
Espèces réactives
human
Technique(s)
immunofluorescence: suitable
western blot: suitable
Isotype
IgG2b
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MAD2L1(4085)
Description générale
Mad2, also known as MAD2, MAD2L1, or Mitotic spindle assembly checkpoint protein MAD2A, is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. Mad2 then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately from cell death or disease.
Immunogène
His-tagged recombinant protein corresponding to human Mad2.
Application
Anti-Mad2 Antibody, clone AS55-A12 is a highly specific mouse monoclonal antibody, that targets MAD2 & has been tested in western blotting & Immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cell lysate (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad2 in PtK1 cells transfected with Alexa-Mad2 (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cells transfected with pCMV expressing wild-type myc-tagged Mad2, and certain mutations of myc-tagged Mad2 (This mutant does not bind to the core of Mad1/Mad2) (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad2 in PtK1 cells transfected with Alexa-Mad2 (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cells transfected with pCMV expressing wild-type myc-tagged Mad2, and certain mutations of myc-tagged Mad2 (This mutant does not bind to the core of Mad1/Mad2) (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).
Qualité
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Mad2 in 10 µg of HEK293 cell lyate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Mad2 in 10 µg of HEK293 cell lyate.
Description de la cible
~23 kDa observed
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Katie L Dale et al.
Journal of cell science, 135(17) (2022-08-06)
Chromosomal instability (CIN), the process of increased chromosomal alterations, compromises genomic integrity and has profound consequences on human health. Yet, our understanding of the molecular and mechanistic basis of CIN initiation remains limited. We developed a high-throughput, single-cell, image-based pipeline
Timothy B Branigan et al.
Cell reports, 34(9), 108808-108808 (2021-03-04)
To identify genes whose loss confers resistance to CHK1 inhibitors, we perform genome-wide CRISPR-Cas9 screens in non-small-cell lung cancer (NSCLC) cell lines treated with the CHK1 inhibitor prexasertib (CHK1i). Five of the top six hits of the screens, MYBL2 (B-MYB)
Thomas Wild et al.
Cell reports, 25(9), 2317-2328 (2018-11-30)
The multisubunit ubiquitin ligase APC/C (anaphase-promoting complex/cyclosome) is essential for mitosis by promoting timely degradation of cyclin B1. APC/C is tightly regulated by the spindle assembly checkpoint (SAC), which involves MPS1 and MAD2-dependent temporal inhibition of APC/C. We analyzed the
Babhrubahan Roy et al.
Current biology : CB, 32(1), 237-247 (2021-12-04)
Accurate chromosome segregation during cell division requires amphitelic chromosome attachment to the spindle apparatus. It is ensured by the combined activity of the spindle assembly checkpoint (SAC),1 a signaling mechanism that delays anaphase onset in response to unattached chromosomes, and
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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