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MABD117

Sigma-Aldrich

Anti-GATA Antibody3, clone 7B5

ascites fluid, clone 7B5, from mouse

Synonyme(s) :

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Clone:
7B5, monoclonal
application:
IHC
WB
Espèces réactives:
human
Technique(s):
immunohistochemistry: suitable
western blot: suitable
citations:

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

7B5, monoclonal

Espèces réactives

human

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GATA3(2625)

Description générale

GATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.

Immunogène

Purified recombinant fragment of human GATA3 expressed in E. Coli.

Application

Detect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Qualité

Evaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.

Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.

Description de la cible

~75 kDa observed

Forme physique

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
GATA3-hIgGFc transfected HEK293 cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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