MABC590

Anti-GRIM-19, clone 1A8 Antibody

clone 1A8, from mouse

• Synonyme(s) : NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, Cell death regulatory protein GRIM-19, Complex I-B16.6, CI-B16.6, Gene associated with retinoic and interferon-induced mortality 19 protein, GRIM-19, Gene associated with retinoic and IFN-ind
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 1A8, monoclonal
• Espèces réactives: mouse, human
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_057049
• Numéro d'accès UniProt: Q9P0J0
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NDUFA13(51079)

Description

Description générale

GRIM-19, also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, Cell death regulatory protein GRIM-19, Complex I-B16.6, CI-B16.6, Gene associated with retinoic and interferon-induced mortality 19 protein, Gene associated with retinoic and IFN-induced mortality 19 protein, NADH-ubiquinone oxidoreductase B16. GRIM-19 6 subunit, is encoded by the gene name NDUFA13, GRIM19, CDA016, CGI-39. GRIM-19 is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I NADH dehydrogenase plays an important role in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. GRIM-19 is also suggested to be involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Additionally, GRIM-19 may play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.GRIM-19 is expressed widely, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In the intestinal mucosa, GRIM-19 is down-regulated in areas involved in Crohn disease and ulcerative colitis. Mutations in GRIM-19 have been associated with Hurthle cell thyroid carcinoma (HCTC), a rare type of thyroid cancer accounting for only about 3-10% of all differentiated thyroid cancers.

Immunogène

Recombinant protein corresponding to human GRIM-19.

Application

Anti-GRIM-19, clone 1A8 | MABC590 is an antibody against GRIM-19 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: A representative lot detected GRIM-19 in MCF-7 and RAW264.7 cell lysate (Hu, J., et al. (2002). J. Interferon & Cytokine Research. 22:1017-1026).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GRIM-19 in MCF-7 cell lysate (Hu, J., et al. (2002). J. Interferon & Cytokine Research. 22:1017-1026).
Immunocytochemistry Analysis: A representative lot detected GRIM-19 in MCF-7 cells (Hu, J., et al. (2002). J. Interferon & Cytokine Research. 22:1017-1026).

Qualité

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GRIM-19 in 10 µg of MCF-7 cell lysate.

Description de la cible

~16 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable