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MABC557

Sigma-Aldrich

Anti-FANCA Antibody, clone 5G9

clone 5G9, from mouse

Synonyme(s) :

Fanconi anemia group A protein, Protein FACA

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

5G9, monoclonal

Espèces réactives

human

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NP_000126

Numéro d'accès UniProt

O15360

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... FANCA(2175)

Description générale

FANCA, also named as FAA, FACA and FANCH, belongs to the multisubunit FA complex which is not found in patients with Fanconi anemia. FANCA may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Mutations in FANCA have been associated with Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies.

Immunogène

KLH-conjugated linear peptide corresponding to the C-terminus of human FANCA.

Application

Anti-FANCA Antibody, clone 5G9 is a highly specific mouse monoclonal antibody, that targets FANCA & has been tested in western blotting & IP.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FANCA in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected FANCA in normal HSC93 and FA mutant lymphoblast whole cell extracts. The same lot also detected FANCA in HeLa whole cell extracts and in FANCA transfected PD430 whole cell extracts (Tremblay, C. S., et al. (2008). Blood. 112(5):2062-2070.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCA from yeast extracts expressing FANCA. The same lot also immunoprecipiated FANCA from 293T whole cell extracts expressing FANCA (Tremblay, C. S., et al. (2008). Blood. 112(5):2062-2070.)

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCA from FANCA wild type FA lymphoblast cell lysate (de Winter, J. P., et al. (2000). Hum Mol Genet. 9(18):2665-2674.).

Qualité

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FANCA in 10 µg of Raji cell lysate.

Description de la cible

~165 kDa observed

Forme physique

Format: Purified

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Anudari Munkhjargal et al.
International journal of molecular sciences, 22(15) (2021-08-08)
Promyelocytic leukemia (PML) protein is the core component of subnuclear structures called PML nuclear bodies that are known to play important roles in cell survival, DNA damage responses, and DNA repair. Fanconi anemia (FA) proteins are required for repairing interstrand
Mafei Xu et al.
Science advances, 5(10), eaax6366-eaax6366 (2019-10-22)
Alternative lengthening of telomeres (ALT) is known to use homologous recombination (HR) to replicate telomeric DNA in a telomerase-independent manner. However, the detailed process remains largely undefined. It was reported that nuclear receptors COUP-TFII and TR4 are recruited to the

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