MABC1227

Anti-PIP4K2B (PIP5K2B) Antibody, clone 7D10.1

clone 7D10.1, from mouse

• Synonyme(s) : Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 7D10.1, monoclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_003550
• Numéro d'accès UniProt: P78356
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PIP4K2B(8396)

Description

Description générale

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC 2.7.1.149; UniProt P78356; also known as 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PIP4K2B, PtdIns(5)P-4-kinase isoform 2-beta) is encoded by the PIP4K2B (also known as PIP5K2B, PIP5KIIB) gene (Gene ID 8396) in human. PIP4K2B catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 5-phosphate to 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It is highly expressed in brain, heart, pancreas, skeletal muscle and kidney, however, lower levels are detected in placenta, lung, and liver. Mice deficient in PIP4K2B are shown to be hypersensitive to insulin and exhibit reduced body weights compared to wild-types. Adult male mice lacking PIP4K2B have significantly less body fat, but female mice show increased sensitivity to insulin in the presence of normal adiposity. Mice lacking PIP4K2B exhibit enhanced in vivo insulin-induced activation of Akt in the skeletal muscle and liver. Ref.: Lamia, KA., et al. (2004). Mol. Cell 24, 5080-5087

Spécificité

Epitope region is present in spliced isoform 1, but not in isoform 2 reported by UniProt (P78356).

Immunogène

GST-tagged recombinant fragment corresponding to a region within the C-terminal half of human PIP4K2B (PIP5K2B).

Application

Detect PIP4K2B using this mouse monoclonal Anti-PIP4K2B (PIP5K2B) Antibody, clone 7D10.1, Cat. No. MABC1227, validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Qualité

Evaluated by Western Blotting in L-929 mouse fibroblast lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIP4K2B (PIP5K2B) in 10 µg of L-929 mouse fibroblast lysate.

Description de la cible

~48 kDa observed. 47.25/47.19 kDa (human/mouse) calculated (Met 1 removed). Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable