MAB8564
Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5
ascites fluid, clone 613-F1-B5-E5, Chemicon®
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
613-F1-B5-E5, monoclonal
Species reactivity:
human
Application:
IF
Citations:
1
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
antibody form
ascites fluid
antibody product type
primary antibodies
clone
613-F1-B5-E5, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
isotype
IgG2a
shipped in
dry ice
Application
Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5 detects level of Poliovirus 3 & has been published & validated for use in IF.
IFA at 1:100-1:400.
Final working dilutions must be determined by end user.
SUGGESTED IFA
PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μL diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μL diluted labeled antibody.
5. Repeat steps 2 and 3.
6. Mount with buffered glycerol.
Final working dilutions must be determined by end user.
SUGGESTED IFA
PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μL diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μL diluted labeled antibody.
5. Repeat steps 2 and 3.
6. Mount with buffered glycerol.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Biochem/physiol Actions
Reacts with the Poliovirus 3.
Preparation Note
Store at -20°C for up to 12 months in convenient, undiluted aliquots. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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