Yes, the same OPN antibody pair is used in all of the Human Milliplex kits (HANG2MAG-12K, HBNMAG-51K, HCCBP1MAG-58K, HKI4MAG-99K, HND3MAG-39K).
HBNMAG-51K
MILLIPLEX® Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay
The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.
Synonyme(s) :
human bone metabolic biomarkers immunoassay panel, luminex human bone metabolism multiplex assay, millipore human bone metabolic biomarkers multiplex kit
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About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Produits recommandés
Niveau de qualité
Espèces réactives
human
Fabricant/nom de marque
Milliplex®
assay range
accuracy: 82-97%
standard curve range: 0.24-1,000 pg/mL
(TNFα)
standard curve range: 1-3,000 pg/mL
(IL-1β)
standard curve range: 1-6,000 pg/mL
(ACTH & IL-6)
standard curve range: 146-600,000 pg/mL
(OC)
standard curve range: 24-100,000 pg/mL
(SOST)
standard curve range: 37-150,000 pg/mL
(FGF-23)
standard curve range: 49-200,000 pg/mL
(Leptin)
standard curve range: 5-20,000 pg/mL
(DKK-1)
standard curve range: 5-20,000 pg/mL
(PTH)
standard curve range: 61-250,000 pg/mL
(Insulin)
standard curve range: 7-30,000 pg/mL
(OPG)
standard curve range: 98-400,000 pg/mL
(OPN)
Technique(s)
multiplexing: suitable
Méthode de détection
fluorometric (Luminex xMAP)
Conditions d'expédition
wet ice
Description générale
Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.
MILLIPLEX® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Bone
MILLIPLEX® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Bone
Spécificité
Cross Reactivty
No cross reactivity between the antibodies for an analyte in this panel were observed.
No cross reactivity between the antibodies for an analyte in this panel were observed.
Application
- Analytes: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteopontin (OPN), Osteoprotegerin (OPG), PTH, Sclerostin (SOST), TNFα
- Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or extracts
- Recommended Sample Dilution: 25 μL per well of d1:2 diluted serum or plasma; cell/tissue culture samples may be used neat or diluted in appropriate control medium
- Assay Run Time: Overnight (16–18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
- Research Category: Metabolism
- Research Subcategory: Bone
Caractéristiques et avantages
Design your multiplex kit by choosing available analytes within this panel.
Liaison
Replaces: HBN1B-51K
Autres remarques
Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitivities.
Informations légales
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Organes cibles
Respiratory Tract
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Javier Diaz-Castro et al.
Food & function, 11(9), 7523-7531 (2020-08-17)
Bone and energy metabolism are profoundly influenced by exercise. The objective of this study was to determine for the first time whether a short-term supplementation with ubiquinol could have a modulating effect on bone turnover and energy metabolism associated with
Ravit Regev et al.
Bone reports, 14, 100747-100747 (2021-01-29)
MCTO is a rare disorder, caused by mutations in the MafB gene, a negative regulator of receptor activator of nuclear factor-кB ligand (RANKL). Manifestations include carpal and tarsal osteolysis and renal failure. Pathophysiology is poorly understood, and no effective treatment
Stephen M Ford et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 311(5), R888-R897 (2016-11-03)
Alcohol use disorders (AUDs) frequently exist among persons living with HIV/AIDS. Chronic alcohol consumption, HIV infection, and antiretroviral therapy (ART) are independently associated with impairments in glucose-insulin dynamics. Previous studies from our laboratory have shown that chronic binge alcohol (CBA)
Chia-Liang Tsai et al.
Experimental physiology, 102(12), 1683-1699 (2017-10-07)
What is the central question of this study? Obesity is linked to cognitive deficits, elevated energy metabolic indices and low-grade systemic inflammation. Do the relationships between neurocognitive performance and the biochemical markers (e.g. energy metabolic indices and inflammatory cytokines) occur
Sharon M Moe et al.
Circulation, 132(1), 27-39 (2015-06-11)
Patients with kidney disease have disordered bone and mineral metabolism, including elevated serum concentrations of fibroblast growth factor-23 (FGF23). These elevated concentrations are associated with cardiovascular and all-cause mortality. The objective was to determine the effects of the calcimimetic cinacalcet
Contenu apparenté
Simplify protein biomarker discovery with the MILLIPLEX® PLEXpedition 115-Plex Multiplex Screening Panel. Effortlessly screen cytokines and other markers with Luminex® technology.
Uncover tips and tricks to multiplexing in this guide from the experts. Learn how you can enhance the power of your research with MILLIPLEX® multiplexing, including industry guidance for immunoassays, multiplexing tips, one-day MILLIPLEX® multiplex assays for limited lab time, and more.
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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Are the OPN antibodies used for detection and capture the same in all Human Milliplex kits?
1 answer-
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What is the specificity of Osteocalcin in the Human Bone Panel, HBNMAG-51K?
1 answer-
The Osteoclacin antibodies were developed using synthetic whole molecule osteocalcins that were gamma carboxylated in the appropriate positions for each species. These antibodies were then affinity purified with peptides that were also gamma carboxylated. The standard material is also carboxylated. However, it is unknown whether the antibodies recognize non-carboxylated or under-carboxylated material, or only carboxylated material, as this has never been tested.
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How can I determine if the Human Bone Milliplex kit is measuring total OC or uncarboxylated OC?
1 answer-
The osteocalcin antibodies were developed using synthetic whole molecule osteocalcins that were gamma carboxylated and then affinity purified with peptides that were also gamma carboxylated. The standard material is also carboxylated. However, the antibodies have not been tested to determine if they recognize non-carboxylated, under-carboxylated, or only carboxylated material.
For the Human Bone panel measuring Osteocalcin, the capture antibody is a monoclonal OC antibody that recognizes intact human osteocalcin and reacts with osteocalcin fragments corresponding to amino acids 15-31 and 20-43 of the native molecule. The detection antibody, on the other hand, is a monoclonal OC antibody that also recognizes intact human osteocalcin and reacts with osteocalcin fragments corresponding to amino acids 1-19, 7-19, and 15-31 of the native molecule.
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