ABS988

Anti-ACAT-2 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : SOAT2_HUMAN, SOAT2, ACACT2, ACTL
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, monkey
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003569
• Numéro d'accès UniProt: O75908
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ACAT2(39)

Description

Description générale

Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT-2), also known as Sterol O-acyltransferase 2
(SOAT2), is a synthetic enzyme that is important in lipoprotein assembly, steroid metabolism, and dietary cholesterol metabolism and absorption. ACAT-2 is expressed primarily in the liver and intestine in enterocytes in their endoplasmic reticulum. ACAT-2 esterifies cholesterol allowing it to be more efficiently packaged and transported. Free cholesterol can be taken up by lipoproteins but it is confined to the outer surface of the lipoparticle. Esterification allows internal packaging and much more efficient transport through the blood stream. Gene expression of ACAT-2 is partially regulated by the transcriptional repressor TGIF1 as well as other factors such as thyroid hormone expression and microRNA miR181d.

Immunogène

Recombinant protein corresponding to Human ACAT-2.

Application

Immunocytochemistry Analysis: A representative lot detected ACAT-2 in human liver tissue (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023).
Western Blot Analysis: A representative lot detected ACAT-2 in human liver lysate (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023)

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

This Anti-ACAT-2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ACAT-2.

Qualité

Evaluated by Western Blotting in human lung tissue lysate using a non-reduced gel.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACAT-2 in 10 µg of human lung tissue lysate using a non-reduced gel

Description de la cible

~ 55 kDa observed, uncharacterized bands may appear at lower molecular weight

Forme physique

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 20 mM NaPO4, 28 mM NaCl and 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1