ABS1645

Anti-Chromatin-Modifying Protein 6

from rabbit

• Synonyme(s) : Chromatin-modifying protein 6, Charged multivesicular body protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, monkey
• Technique(s): immunofluorescence: suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_078867
• Numéro d'accès UniProt: Q96FZ7
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CHMP6(79643)

Description

Description générale

Charged multivesicular body protein 6 (UniProt: Q96FZ7; also known as Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6) is encoded by the CHMP6 (also known as VPS20) gene (Gene ID: 79643) in human. CHMP6 is a human orthologue of yeast Vps (vacuolar protein sorting) 20, a component of ESCRT (endosomal sorting complex required for transport)-III. Various CHMP6 orthologues in organisms ranging from yeast to humans contain the N-myristoylation consensus sequence at each N-terminus. CHMP6 is an ubiquitously expressed protein that has an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP6 is probable core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins.

Spécificité

This polyclonal antibody detects chromatin-modifying protein 6 (CHMP6) in human and monkey cells.

Immunogène

Epitope: unknown

GST-tagged full length recombinant human Chromatin-modifying protein 6.

Application

Anti-Chromatin-Modifying Protein 6, Cat. No. ABS1645, is a rabbit polyconal antibody that is used to detect Chromatin-Modifying Protein 6 and has been tested for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in HEK293 cell lysate (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

Research Category
Signaling

Qualité

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 6 in 10 µg of HEK293 cell lysate.

Description de la cible

~32 kDa observed; 23.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1