ABE239

Anti-JARID1B Antibody

serum, from rabbit

• Synonyme(s) : JMJD, lysine (K)-specific demethylase 5B, Jumonji, AT rich interactive domain 1B (RBP2-like), putative DNA/chromatin binding motif, jumonji, AT rich interactive domain 1B, Histone demethylase JARID1B, retinoblastoma-binding protein 2, homolog 1A, Cancer/
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_006609
• Numéro d'accès UniProt: Q9UGL1
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KDM5B(10765)

Description

Description générale

JARID1B (jumonji AT rich interactive domain 1B), also known as KDM5B, is histone demethylase with a preference for mono, di, and trimethylated lysine 4 of histone H3. Demethylation of lysine 4 in histone 3 is commonly associated with transcriptional repression. This nuclear protein belongs to the ARID family of DNA binding proteins and is highly expressed in a large number of breast cancers. This protein has been linked to the repressinion of multiple tumor suppressor genes including BRCA1 and HOXA5.

Spécificité

This antibody recognizes JARID1B at the C-terminal domain.

Immunogène

Epitope: C-terminal domain

GST-tagged recombinant protein corresponding to human JARID1B at the C-terminal domain.

Application

Anti-JARID1B Antibody is a Rabbit Polyclonal Antibody for detection of JARID1B also known as lysine (K)-specific demethylase 5B & has been validated in WB & IP.

Immunoprecipitation:
A representative lot of this antibody was reported to work for immunoprecipitation analysis in Saos-2, MCF-7, and T47D lysates (Laboratory of E. Benevolenskaya , University of Illinois at Chicago).

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Histone Modifying Proteins

Qualité

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected JARID1B on 10 µg of A431 cell lysate.

Description de la cible

~180 kDa observed. Uncharacterized bands may be observed at ~ 48 and 53 kDa in some lysates.

Forme physique

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
A431 cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1