ABC110

Anti-ELMO1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Engulfment and cell motility protein 1, Protein ced-12 homolog
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41
• Clone: polyclonal
• application: WB
• Espèces réactives: human
• Technique(s): western blot: suitable
• citations: 1

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_055615
• Numéro d'accès UniProt: Q92556
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ELMO1(9844)

Description

Description générale

Engulfment and cell motility protein 1 (ELMO1) is a cytoplasmic protein involved in the clearance of apoptotic cells by phagocytosis. ELMO1 is activated downstream of the phosphatidylserine receptor BAI1. ELMO1 binds to DOCK180 and facilitates its transport to the plasma membrane where DOCK180 activates the GTPase RAC1, possibly via Arf6. RAC1, like other small GTPases, mediate cytoskeleton remodeling and cell motility which is necessary for the internalization of target cells.

Spécificité

No homology to Rat was found.

This antibody recognizes ELMO1 at the ELMO domain.

Immunogène

Epitope: ELMO domain

KLH-conjugated linear peptide corresponding to human ELMO1 at the ELMO domain.

Application

Detect ELMO1 using this Anti-ELMO1 Antibody validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Qualité

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ELMO1 on 10 µg of Jurkat cell lysate.

Description de la cible

~78 kDa observed. This antibody exhibits strong affinity, high dilutions are recommended. The calculated molecular weight is 84 kDa, however ELMO1 has been shown as a ~78 kDa band in western blots (Gumienny, T.L., et al. (2001). Cell. 107:27–41.)

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Jurkat cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable