616430

TGF-α, Human, Recombinant, E. coli

• Synonyme(s) : TGF-α, Human, Recombinant, E. coli, Transforming Growth Factor-α
• Numéro CAS: 105186-99-0
• Numéro MDL: MFCD00082077
• Code UNSPSC : 12352202

Propriétés

• Essai: ≥97% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤100 pg/μg Endotoxin (pg/μg TGF-α)
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

Actions biochimiques/physiologiques

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 30% acetonitrile, 0.1% TFA.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

Autres remarques

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany