567693

SOST, Mouse, Recombinant

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Pureté: ≥90% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: <1.0 EU/μg protein Endotoxin
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Recombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.

Actions biochimiques/physiologiques

Inhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 0.2 µm filter-sterilized PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Autres remarques

Kusu, N. et al. 2003. J. Biol. Chem.278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet.10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet.68, 577.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3